Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MFQIGDNIVYPMHGAGIIEAIEEKEFLEEKQQYYVIRMSISNMTVMIPTSKILSSNIRPVTDILALKHIMHIFQHGESDRLLPWKQRYKINTDKIKTGEIQEGAEVVRDLMRMKKEKALNASEKKMLDNAYEFLISELEVIKGITEKQIKSFG 4XLR Chain:M ((4-154)) -FRPGDKVVLPPYGVGVVAGIAQRSVSGVSRAYYQVDFPGSRSKAYVPVEAPHSVGLRKALAPEEVPVILDLLKNGRMPLPKQWAARHRKTSEILADGNPYRIAQMAGQLRAWEVERGLPDLDRQALRRAIHLLAEEVAQSLEITVQEAKRL-

General information: TITO was launched using: RESULT: Template: 4XLR.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain M - contact count / total energy / energy per contact / energy per residue : 579 -57588 -99.46 -381.38 target 2D structure prediction score : 0.52 Monomeric hydrophicity matching model chain M : 0.71 3D Compatibility (PKB) : -99.46 2D Compatibility (Sec. Struct. Predict.) : 0.52 1D Compatibility (Hydrophobicity) : 0.71 QMean score : 0.417

(partial model without unconserved sides chains): PDB file : Tito_4XLR.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4XLR-query.scw PDB file : Tito_Scwrl_4XLR.pdb: