Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MLIILVMFLLGIILGFIGAGGAGFVIALLTLLFHIPIHTALGTSLAGMAFTSLSGAYSHYREGNIQMKIGLIVGGFAAVGSFFGAKLTSFIPADLLHYLTA-----GMLFLSAILILIRLFILKEKAQVNQSTLSTYTRAVILGI-AAGVLSGTFGIGSAPFIQIGLMIMLNLSIRHSVGTTMLVIIPLAVGGGIGYITEGFVDYVLLVKVLVG--TMCGAYVGAKFTNLMPKVVLKSAIFLTPAIAGLLLLF 4QQD Chain:A ((133-281)) ------------------------------------------------------------------------------------------GLYKVNEYVDARDTNMGAWFEAQVVRVTR----KEEDVIYHVKYDDYPENGVVQMNSRDVRARARTIIKWQDLEVGQVVMLNYNPDNPKER------GFWYDAEISRKRETRTARELYANVVLGDDSLNDCRIIFVDEVFKIE--------------------

General information: TITO was launched using: RESULT: Template: 4QQD.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 550 -81060 -147.38 -648.48 target 2D structure prediction score : 0.16 Monomeric hydrophicity matching model chain A : 0.49 3D Compatibility (PKB) : -147.38 2D Compatibility (Sec. Struct. Predict.) : 0.16 1D Compatibility (Hydrophobicity) : 0.49 QMean score : 0.007

(partial model without unconserved sides chains): PDB file : Tito_4QQD.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4QQD-query.scw PDB file : Tito_Scwrl_4QQD.pdb: