Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MLNIKSDRIMTSDPQKFTVDGVRFF---QQYLNKIRPDAMEIDNLIINSDIIKEDLIVHVSKMI----DRLALYDLHRKRSIDKHFSAAFTVDINDREEFYSANIADVSIDLNESSGV 4P04 Chain:A ((64-126)) -----------------TYDGVPIFGLYQKFANKVTVEWKE------NGKVMKDDYVVHTSAIVNNYMDNRSISDLQQTKVI------------------------------------

General information: TITO was launched using: RESULT: Template: 4P04.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 75 -4495 -59.93 -86.44 target 2D structure prediction score : 0.06 Monomeric hydrophicity matching model chain A : 0.66 3D Compatibility (PKB) : -59.93 2D Compatibility (Sec. Struct. Predict.) : 0.06 1D Compatibility (Hydrophobicity) : 0.66 QMean score : -0.044

(partial model without unconserved sides chains): PDB file : Tito_4P04.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4P04-query.scw PDB file : Tito_Scwrl_4P04.pdb: