Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTENFWRELPRPFFVLAPMEDVTDVVFRHVVSEAGRPDVFFTEFTNS----------ESYCHPDGKDSVRGRLTFTEDEQPIVAHIWGDKPENFRKMSIGMAELGFKGLDINMGCPVPNVAGNGKGSGLICRPAVAAELI-QAAKA------GGLPVSVKTRLGYTDVDEWREWLTHILKQDIANLSIHLRTRAEMSKVD-AHWELIPEIKKLRDEVAPDTLLTINGDIPDRQTGLKLAEQYGVDGIMIGRGIFTNPFAFEKEPKEHSSKELLDLLRLHLDLHDEYSKEEARPYKPLPRFFKIYLRGFRGASELRNQCMNTKSTDEVRALLDDFERKYLDGIE 4BF9 Chain:A ((26-254)) -------------VLLAPMEGVLDSLVRELLTEVNDYDLCITEFVRVVDQLLPVKVFHRIC-PELQNASR-----TPSGTLVRVQLLGQFPQWLAENAARAVELGSWGVDLNCGCPSKTVNGSGGGATLLKDP----ELIYQGAKAMREAVPAHLPVSVKVRLGWDSGEKKFEIADAVQQAGATELVVHGRTKEQGYRAEHIDWQAIGDIRQRL-----NIPVIANGEIWDWQSAQQCMAISGCDAVMIGRGALNIP--------------------------------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 4BF9.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1192 -109714 -92.04 -519.97 target 2D structure prediction score : 0.67 Monomeric hydrophicity matching model chain A : 0.69 3D Compatibility (PKB) : -92.04 2D Compatibility (Sec. Struct. Predict.) : 0.67 1D Compatibility (Hydrophobicity) : 0.69 QMean score : 0.498

(partial model without unconserved sides chains): PDB file : Tito_4BF9.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4BF9-query.scw PDB file : Tito_Scwrl_4BF9.pdb: