Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNIQDMILTLQKHWSSQGCVLMQAYDVEK---------GAGTMSPYTFL-RSIGPEPW-KVAYVEPSRRPADG-RYGENPNRLYQHHQFQVIIKPSPD----------NIQELYLDSLRALGIDPLEHDIRFVEDNWENPSLGCAGLGWEVWLDGMEITQFTYFQQVG---------GLECKP---VSVEITYGIERLASYIQDKENVFDLEWTSGFTVKDLFMMAEYEHSVYTFETSDVDMLFQLFSTYEKEAIKQMDNGLVHPAYDYVLKCSHTFNLLDAKGAISVTERTGYIARVRNLARKVAKTYYEEREKLGFPMLKGEGSSHE 2ZTG Chain:A ((60-266)) FELDEMREYYLNFFERRGHGRIERYPVVARWRTDIYLTIASIADFQPFVTSGVAPPPANPLTISQPCIRLDDLDSVGRT---GRHLTLFEMMAHHAFNYPGKEIYWKNETVAYCTELLNELGVKKE--DIVYKEEPWAG--GGNAGPCLEAIVGGLEVATLVFMNLEEHPEGDIEIKGARYRKMDNYIVDTGYGLERFVWASKGTPTVYDAIFP-------------------------------------------------------------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 2ZTG.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 716 -8183 -11.43 -47.30 target 2D structure prediction score : 0.64 Monomeric hydrophicity matching model chain A : 0.63 3D Compatibility (PKB) : -11.43 2D Compatibility (Sec. Struct. Predict.) : 0.64 1D Compatibility (Hydrophobicity) : 0.63 QMean score : 0.370

(partial model without unconserved sides chains): PDB file : Tito_2ZTG.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2ZTG-query.scw PDB file : Tito_Scwrl_2ZTG.pdb: