Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKEARKRMVKRAVQEIKDGMNVNLGIGMPTLVANEIPDGVHVMLQSENGLLGIGPYPLEGTEDADLINAGKETITEVTGASYFDSAESFAMIRGGHIDLAILGGMEVSEQGDLANWMIPGKMVKGMGGAMDLVNGAK-RIVVIMEHVNKHGESKVKKTCSLPLTGQKVVHRLITDLAVFDFVNGR-MTLTELQDGVTIEEVYEKTEADFAVSQSVLNS 2NRC Chain:A ((263-474)) ----RERIIKRAALEFEDGMYANLGIGIPLLASNFISPNMTVHLQSENGILGLGPYPLQNEVDADLINAGKETVTVLPGASYFSSDESFAMIRGGHVNLTMLGAMQVSKYGDLANWMIPGKLVKGMGGAMDLVSSAKTKVVVTMEHSAKGNAHKIMEKCTLPLTGKQCVNRIITEKAVFDVDRKKGLTLIELWEGLTVDDIKKSTGCDFAVSPKLI--

General information: TITO was launched using: RESULT: Template: 2NRC.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1095 -52837 -48.25 -251.60 target 2D structure prediction score : 0.47 Monomeric hydrophicity matching model chain A : 0.87 3D Compatibility (PKB) : -48.25 2D Compatibility (Sec. Struct. Predict.) : 0.47 1D Compatibility (Hydrophobicity) : 0.87 QMean score : 0.550

(partial model without unconserved sides chains): PDB file : Tito_2NRC.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2NRC-query.scw PDB file : Tito_Scwrl_2NRC.pdb: