Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MAKKEFKAESKRLLDMMINSIYTQKEIFLRELISNSSDAIDKIYYKALTDDALTFDKDSYYIKVAADKDARTLTISDTGIGMTKDELEQHLGTIAKSGSLAF--KKENELKDGHDIIGQFGVGFYAAFMVADVVTVISK-ALGSEEAYKWESAGADGYTIEPCEKDSVGTDIILKIKENTEDDSYDEFLEEYRLKAIIKKYSDFIRYPIKMDTTINKPKEGSENEFEEVQEEQTVNSMVPIWRKNKSELTDEDYEKFYAEKHYGFDKPLTHVHISVDGAVRYNAILFVPENMPFDYYSKEYEKGLELYSNGVLIMNKCADLLPDHFSFVKGMVDSEDLSLNISREMLQHDRQLKLIAKNISKKIKSELQSLLKKDREKYETFYKSFGRQLKFGVYNDFGAN-KDQLKDLLLFYSS--KEKKLVSLDEYVSRMPEEQKFIYYATGDSYDRIEKLPQTEMVADKGYEILYFTEDIDEFAIKMLASYQEKEFKSVSSGDLGIDTDEDQKQSEEEES--KYKDLFEEMKNILSDKVKNVRISKRLKSHPVCLAADGEVTIEMEKILNAMPDSQNVKA---EKVLEINPNHEVFQTLQDAFEQDKEKLSLYTNLLYNQALLIEGLPIQDPVEFTNNICKVMV 4J0B Chain:A ((29-641)) -SKHEFQAETKKLLDIVARSLYSEKEVFIRELISNGSDALEKLRHRMITAGG---DTAPMEIHLQTDSVKGTFTIQDTGVGMNKEDLVSNLGTIARSGSKAFLDALQNQAEASSSIIGQFGVGFYSAFMVADKVEVYSQSAEADAPGYKWSSDGSGVFEVAEASGVRQGTKIVLHLK-----DDCKEFSSEDRVKEVVTKYSNFVSFPIFLNG-------------------RRLNTLQALWMMEPKDISEWQHEEFYRYVAQAYDKPRYTLHYRADAPLNIRSIFYVPEMKP-------MGSSVALYSRKILIQTKATDILPKWLRFLRGVVDSEDIPLNLSRELLQESALIRKLRDVLQQRVIRFLLDQSKKDPEKYARFFEDYGLFMREGIVTTGEQSVKEDIAKLLRFESSALPAGQQTSLMEYSSRMKAGTRNIYYLCAPNRHLAEHSPYFEAMK--DMEVLFCFEQFDELTLLHLREFDRKKLISAET----------------RLSSEQAEDLLAWMRNALVQRVTNIKVTPRLDTHPAMI-----TVLEMGAARHFLRTQ-----QILQPTLEINTGHDLIKKLHALKDSNPELAQLLLEQIYDNAMIAAGLN-EDPRPMISRLNQLLT

General information: TITO was launched using: RESULT: Template: 4J0B.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 2778 42179 15.18 75.05 target 2D structure prediction score : 0.56 Monomeric hydrophicity matching model chain A : 0.77 3D Compatibility (PKB) : 15.18 2D Compatibility (Sec. Struct. Predict.) : 0.56 1D Compatibility (Hydrophobicity) : 0.77 QMean score : 0.516

(partial model without unconserved sides chains): PDB file : Tito_4J0B.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4J0B-query.scw PDB file : Tito_Scwrl_4J0B.pdb: