Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTVLWVAAVIALACLNVIQFIMKKKRDGNLAYTADQLSYMLSRDSAGQILLPTDDHALKDLLVNINLIVENRQQISAQFAKTEQSMKRMLTNMSHDLKTPLTVILGYIEAIQSDPNMPD-EERERLLGKLRQKTNELIQMINSFFDLAKLESEDKEIPITKVHMNDICKR--NILHYYDAVQSKGFQAAIDIPDTPVYAQANEEALDRILQNLLSNAIQYG---AAGKLIGLTLSYDERNIAITVWDRGKGISETDQQRVFERLYTLEESRNKAFQGSGLGLTITKRLTEKMGGIISVQSKPYERTAFTITLKRMTY 4JAV Chain:A ((23-247)) ----------------------------------------------------------------------------------------FIANISHELRTPLTAIKAYAETIYNSLGELDLSTLKEFLEVIIDQSNHLENLLNELLDFSRLERKSLQINREKVDLCDLVESAVNAIKEFASSHNVNVLFESNVP-CPVEAYIDPTRIRQVLLNLLNNGVKYSKKDAPDKYVKVILDEKDGGVLIIVEDNGIGIPDHAKDRIFEQFYRVDSSLTYEVPGTGLGLAITKEIVELHGGRIWVESEVGKGSRFFVWIPK---

General information: TITO was launched using: RESULT: Template: 4JAV.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 886 -104540 -117.99 -477.35 target 2D structure prediction score : 0.74 Monomeric hydrophicity matching model chain A : 0.68 3D Compatibility (PKB) : -117.99 2D Compatibility (Sec. Struct. Predict.) : 0.74 1D Compatibility (Hydrophobicity) : 0.68 QMean score : 0.407

(partial model without unconserved sides chains): PDB file : Tito_4JAV.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4JAV-query.scw PDB file : Tito_Scwrl_4JAV.pdb: