Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MEHLPEQYRQLFPTLQTHTMLASCSQSALAEPVSRAIQDYY---DSLLYKGTNW--KEAIEKTEFARNEFAKLIGA-EPDEVAIVPSVSDALVSVASSLTAFGKK---HVVYTDMDFPAVPHVWQAHSDYTVSVIPSI----DGVLPLEQYETHISDETVLTCVPHVHYRDGYVQDIKAIAEISQRKGSLLFVDAYQSAGHIPIDVKEWGVDMLAAGTRKYLLGIPGVAFLYVRKELADALKPKASAWFGRESGF--DGAYAKVARRFQTGTPAFISVYAAAAALSLLNHIGVSHIRDHVKTICADAVQYAAEKGLQLAAAQGGIQPGMVAIRDERASETAGLLKKKKVICAPRENVIRLAPHFYNTKEEMRHAIDEIAAKTIHK 4Q75 Chain:A ((42-320)) --------------------LDSAATSQKPAAVLDALQNYYEFYNSNVHRGIHYLSAKATDEFELARKKVARFINASDSREIVFTRNATEAINLVAYSWGLSNLKPGDEVILTVAEHHSCIVPWQIVSQKTGAVLKFVTLNEDEVPDINKLRELISPKTKLVAVHHVSNVLASSLPIEEIVVWAHDVGAKVLVDACQSVPHMVVDVQKLNADFLVASSHK-MCGPTGIGFLYGKSDLLHSMPPFLGGGEMISDVFLDHSTYAEPPSRFEAGTPAIGEAIALGAAVDYLSGIGMPKIHEY--------------------------------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 4Q75.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1451 -196307 -135.29 -746.41 target 2D structure prediction score : 0.62 Monomeric hydrophicity matching model chain A : 0.71 3D Compatibility (PKB) : -135.29 2D Compatibility (Sec. Struct. Predict.) : 0.62 1D Compatibility (Hydrophobicity) : 0.71 QMean score : 0.461

(partial model without unconserved sides chains): PDB file : Tito_4Q75.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4Q75-query.scw PDB file : Tito_Scwrl_4Q75.pdb: