Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MVNKQLKKKAQEIGNVPTHYELEIEDYDQKQKKYGQAYFIWKDPKDPEKHITVELRNDGALLTFSTTVHSETDKKLPDAELKLTALQFAAANHPGTFMNFHFQGKEERGQHIRFVYTKMELGLPIPNSGFLIDMTRSGQIVHFLYYGEGHKAEVPTEFVAKEKVVSHYLNTMSFELMYDVIDGEQEPRLVYEPILPGYSYPADVDEIVPDQHIADERIENTAPLPPLQNKEEVDIFALLGFTSD--MQKVSERDFGEQIGSTWRRGAAPERKDLSIGSYFETRNKNTIKMKTDKRTGKLKAALSFMDWRNNLQCSTEECQEIALQFLYALYPRAAEFFRVNPVRIDERGR-VRNHFSVW-YKGVPLRFGAARIIVNPETGLIDAFMAPDIE--PEQLEAINHRPDVSAEEAKEAFLAAFDVK---LEWQPDFTAGSDQHCKLVYKPVY-------PSYIDAHIRKKKRL 5BOI Chain:A ((14-226)) -------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------VAKQVARSFLNLKGNEQI-HIVKSG-KDADYEVYSLTI------------TDPKTNQETYMDITQKGGYPLWVLEDRD-IKKQNISLNDAMNKATKFLKDHRFE---SLVMAESA--QYDNMGVFTFVEQTESGVRIYPDSVKMKMSLEDGSVIGFSAKDFLLKHRTRD--IPKPKISKEQAKTKLNSNVKVMEERLAIITND----LNEEVLCYEFLGTIKNDTYRIFINADTGFEEK-

General information: TITO was launched using: RESULT: Template: 5BOI.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 879 -65129 -74.09 -330.60 target 2D structure prediction score : 0.53 Monomeric hydrophicity matching model chain A : 0.58 3D Compatibility (PKB) : -74.09 2D Compatibility (Sec. Struct. Predict.) : 0.53 1D Compatibility (Hydrophobicity) : 0.58 QMean score : 0.334

(partial model without unconserved sides chains): PDB file : Tito_5BOI.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5BOI-query.scw PDB file : Tito_Scwrl_5BOI.pdb: