Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MIKSWKPQELSISYHQFTVFQKDSTPPVMDWTDEAIEKGYAAADGAISFEAQRNTKAFILFRLNSSETVNSYEKKVTVPFHVTEN-GIHIESI-----MSKRLSFDLPKGDYQLTCWTVPAEMSDLHADTYIIDAVSV 2AEV Chain:A ((275-364)) ---------------------------------EKLNKELKAIDDNINIVYERTPTGFVIKRVYKDDTIN-IKKLIEIGFNLLKNYGIITITVAGMPGASKSLRIDLTSRD---------AERID---DNYIIKAI--

General information: TITO was launched using: RESULT: Template: 2AEV.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 278 -31390 -112.91 -373.68 target 2D structure prediction score : 0.36 Monomeric hydrophicity matching model chain A : 0.65 3D Compatibility (PKB) : -112.91 2D Compatibility (Sec. Struct. Predict.) : 0.36 1D Compatibility (Hydrophobicity) : 0.65 QMean score : 0.267

(partial model without unconserved sides chains): PDB file : Tito_2AEV.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2AEV-query.scw PDB file : Tito_Scwrl_2AEV.pdb: