Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MFHISTLDQIKIAYIGGGSQGWARSLMSDLSIDERMS-GTVALYDLDFEAAQKNE--VIGN---HSGNGRWRYEAVSTLKKALSAADIVIISILP--------GSLDDMEVDVHLPERCGIY-----QSVGDTVGPGGIIRGLRAVPIFAEIARAIRDYAPESWVINYTNPMSVCTRVLYKVFPGIKAIGCCHEVFGTQKLLAEMVTERLGIEVPRREDIRVNVLGINHFTWITKASYRHIDLLPIFREFSAHYGESGYELEGECWRDSVFCSAHRVAFDLFETYGAIPAAGDRHLAEFLPGPYLKQPEVWKFHLTPISF---RKQDRAEKRQETERLI-----VQQRGVAE------------KASGEEGVNIIAALLGLGELVTNVNMPNQGQVLNLPIQAIVETNAF-----ITRNRVQPILSGALPKGVEMLAARHISNQEAVADAGLTKDTGLAFQAFLNDPLVQIDRSDAEQLFNDMLQCIMQS 1VJT Chain:A ((14-451)) ----------KISIIGAGSVRFALQLVGDIAQTEELSREDTHIYMMDVHERRLNASYILARKYVEELNSPVKIVKTSSLDEAIDGADFIINTAYPYDPRYHDSGSQRWDEV-TKVGEKHGYYRGIDSQELNMVSTYTYVLSSYPDMKLALEIAEKMKKMAPKAYLMQTANPVFEITQAV-RRWTGANIVGFCHGVAGVYE-----VFEKLDLDP---EEVDWQVAGVNHGIWLNRFRYRGEDAYPLLDEWIE---KKLPEWEPKNPWDTQMSPA---AMDMYKFYGMLPIGDTVRNGSWKYHYNLETKKKWFGKFGGIDNEVERPKFHEQLRRARERLIKLAEEVQQNPGMKLTEEHPEIFPKGKLSGEQHIPFINAIANNKRVRLFLNVENQGTLKDFPDDVVMELPVWVDCCGIHREKVEPDLTHRI--KIFYLWPR-ILRMEWNLEAYISRDRKVLEEILIRDP-----------------------

General information: TITO was launched using: RESULT: Template: 1VJT.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 2066 -204714 -99.09 -519.58 target 2D structure prediction score : 0.57 Monomeric hydrophicity matching model chain A : 0.73 3D Compatibility (PKB) : -99.09 2D Compatibility (Sec. Struct. Predict.) : 0.57 1D Compatibility (Hydrophobicity) : 0.73 QMean score : 0.372

(partial model without unconserved sides chains): PDB file : Tito_1VJT.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1VJT-query.scw PDB file : Tito_Scwrl_1VJT.pdb: