Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTASQQQIQLARRPQGIPVHEDFRFETIP----VPEPKQGEVLVKTLYVSVDPYMRGRMQDTKSYVEPFALDKALSGGVIAEVVSDGN-HLKKGDIVIGNLSWQEFSAVSESALRKIDTSLAPASAYLGILGMTGLTAYFGLLDIGRPKEGETVVVSGAAGAVGSTVGQIAKIKGARVVGIAGSDEKIDYLKQELQFDEAINYKTADDIQKALQNACPDGVDVYFDNVGGPISDAVMNLLNEFARIPVCGAISSYNAESEADDMGPRVQSKLIKTKSLMQGFIVSDYSDRFSEGAKQLAEWLKAGKLHYEETITEGFENIPDAFLGLFKGENKGKQLIKVSDPS 4HFM Chain:A ((5-341)) --VSNKQVILKNYVTGYPKESDMEIKNVTIKLKVPE-GSNDVVVKNLYLSCDPYMRSRMRK-----ESFAPGSPITGYGVAKVLESGDPKFQKGDLVWGMTGWEEYSIITP-TLFKIHDKDVPLSYYTGILGMPGMTAYAGFHEVCSPKKGETVFVSAASGAVGQLVGQFAKMLGCYVVGSAGSKEKVDLLKSKFGFDEAFNYKEEQDLSAALKRYFPDGIDIYFENVGGKMLDAVLVNMKLYGRIAVCGMISQYNLEQTE---GVHNLFCLITKRIRMEGFLVFDYYHLYPKYLEMVIPQIKAGKVVYVEDVAHGLESAPTALVGLFSGRNIGKQVVMVS---

General information: TITO was launched using: RESULT: Template: 4HFM.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1940 -189505 -97.68 -584.89 target 2D structure prediction score : 0.56 Monomeric hydrophicity matching model chain A : 0.81 3D Compatibility (PKB) : -97.68 2D Compatibility (Sec. Struct. Predict.) : 0.56 1D Compatibility (Hydrophobicity) : 0.81 QMean score : 0.534

(partial model without unconserved sides chains): PDB file : Tito_4HFM.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4HFM-query.scw PDB file : Tito_Scwrl_4HFM.pdb: