Modeling by threading (Tito software)
Unconserved sides chains calculation (Scwrl software)
Evaluation (QMean software)



Input alignment information:
Query sequenceMTASQQQIQLARRPQGIPVHEDFRFETIP----VPEPKQGEVLVKTLYVSVDPYMRGRMQDTKSYVEPFALDKALSGGVIAEVVSDGN-HLKKGDIVIGNLSWQEFSAVSESALRKIDTSLAPASAYLGILGMTGLTAYFGLLDIGRPKEGETVVVSGAAGAVGSTVGQIAKIKGARVVGIAGSDEKIDYLKQELQFDEAINYKTADDIQKALQNACPDGVDVYFDNVGGPISDAVMNLLNEFARIPVCGAISSYNAESEADDMGPRVQSKLIKTKSLMQGFIVSDYSDRFSEGAKQLAEWLKAGKLHYEETITEGFENIPDAFLGLFKGENKGKQLIKVSDPS
4HFM Chain:A ((5-341))--VSNKQVILKNYVTGYPKESDMEIKNVTIKLKVPE-GSNDVVVKNLYLSCDPYMRSRMRK-----ESFAPGSPITGYGVAKVLESGDPKFQKGDLVWGMTGWEEYSIITP-TLFKIHDKDVPLSYYTGILGMPGMTAYAGFHEVCSPKKGETVFVSAASGAVGQLVGQFAKMLGCYVVGSAGSKEKVDLLKSKFGFDEAFNYKEEQDLSAALKRYFPDGIDIYFENVGGKMLDAVLVNMKLYGRIAVCGMISQYNLEQTE---GVHNLFCLITKRIRMEGFLVFDYYHLYPKYLEMVIPQIKAGKVVYVEDVAHGLESAPTALVGLFSGRNIGKQVVMVS---


General information:
TITO was launched using:
RESULT:

Template: 4HFM.pdb
Alignment : align.pir
Tito was launched with SMD and SCWRL
Tito text output
Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1940 -189505 -97.68 -584.89
target 2D structure prediction score : 0.56
Monomeric hydrophicity matching model chain A : 0.81

3D Compatibility (PKB) : -97.68
2D Compatibility (Sec. Struct. Predict.) : 0.56
1D Compatibility (Hydrophobicity) : 0.81
QMean score : 0.534

(partial model without unconserved sides chains):
PDB file : Tito_4HFM.pdb:





(Unconserved sides chains are recalculated) :
Sequence: align-4HFM-query.scw
PDB file : Tito_Scwrl_4HFM.pdb: