Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKEIKEAYQQCGQIVGEYAPACFKALSYLPLKQRQASWAVLSFCHTAAS--------------------------------ADEKVLPAFEAKADHVYQRTNNGKQHLWKAFDHAYRTFTLESEPFREFIAAQKEDAKPYDDLDELLMYAYRTGGAAGLMLLPILTRRKQDQLKQAAVSLGLAIQLVRFLSDLGTDQQKNRI--PRQVMQQFGYTEADLQKGTVNKAFTMTWEYIAFEAEAYLEECQDALPLFPQYSQKTVKAALHLHRAVLEKIRAKQHDVFQYHFALTETEVKQILSDI 3ACX Chain:A ((14-262)) -------FKYCHKIMKKHSKSFSYAFDLLPEDQRKAVWAIYAVCRKIDDSIDVYGDIQFLNQIKEDIQSIEKYPYEYHHFQSDRRIMMALQ----HVAQHKNIAFQSFYNLIDTVYK-----DQHFTMF----ETDA-------ELFGYCYGVAGTVGEVLTPILSDHETHQTYDVARRLGESLQLINILRDVGEDFENERIYFSKQRLKQYEVDIAEVYQNGVNNHYIDLWEYYAAIAEKDFRDVMDQIKVFSIEAQPIIELAARIYIEILDEVR-------------------------

General information: TITO was launched using: RESULT: Template: 3ACX.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 915 -135658 -148.26 -630.97 target 2D structure prediction score : 0.73 Monomeric hydrophicity matching model chain A : 0.71 3D Compatibility (PKB) : -148.26 2D Compatibility (Sec. Struct. Predict.) : 0.73 1D Compatibility (Hydrophobicity) : 0.71 QMean score : 0.598

(partial model without unconserved sides chains): PDB file : Tito_3ACX.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3ACX-query.scw PDB file : Tito_Scwrl_3ACX.pdb: