Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MLENIKQTITRWDERNPWTNVYGLARSIIALSSLLTLLINHPSLIMKPASGISSYPACKMNLSLFCLGENNYMMLNLFRWVCIAILVLVVIGWRPRITGVLHWYVSYSLQSSLIVIDGGEQAAAVMTFLLLPITLTDPRKWHWSTRPIEGKRTLGKITAFISYFVIRIQVAVLYFHSTVAKLSQQEWVDGTAVYYFAQEKTIGFNGFFQALTKPIVTSPFVVIPTWGTLLVQIVIFAALFAPKKHWRLILIIAVFMHEIFAVMLGLISFSIIMAGILILYLTPIDSTIQFTYIRRLLWNKKHKKGEVSV 4C2V Chain:C ((3-20)) ----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------PAWASGNLLTQAIRQQYY-----------------------------------------------------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 4C2V.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain C - contact count / total energy / energy per contact / energy per residue : 9 -2513 -279.17 -139.58 target 2D structure prediction score : 0.33 Monomeric hydrophicity matching model chain C : 0.39 3D Compatibility (PKB) : -279.17 2D Compatibility (Sec. Struct. Predict.) : 0.33 1D Compatibility (Hydrophobicity) : 0.39 QMean score : 0.054

(partial model without unconserved sides chains): PDB file : Tito_4C2V.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4C2V-query.scw PDB file : Tito_Scwrl_4C2V.pdb: