Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSKHNWTLETQLVHNPFKTDGGTGAVSVPIQHASTFHQSSFEEFGA--YDYSRSGTPTRTALEETIAALEGGTRGFAFSSGMAAISTAFL-LLSQGDHVLVTEDVYGGTFRMVTEVLTRFGIEHTFVDMTDRNEVARSIKPNTKVIYMETPSNPTLGITDIKAVVQLAKENGCLTFLDNTFMTPALQRPLDLGVDIVLHSATKFLSGHSDVLSGLAAVKDEELGK-QLYKLQNAFGAVLGVQDCWLVLRGLKTLQVRLEKASQTAQRLAEFFQKHPAVKRVYYPGLADHPGAETHKSQSTGAGAVLSFELESK-EAVKKLVENVSLPVFAVSLGAVESILSYPATMSHAAMPKEEREKRGITDGLLRLSVGVEHADDLEHDFEQALKEIAPVSVR 3MKJ Chain:A ((7-395)) -----YGFNTQIVHAGQQPDPSTGALSTPIFQTSTFVFDSAEQGAARF-----LGNPTTDALEKKLAVLERGEAGLATASGISAITTTLLTLCQQGDHIVSASAIYGCTHAFLSHSMPKFGINVSFVDAAKPEEIRAAMRPETKVVYIETPANPTLSLVDIETVAGIAHQQGALLVVDNTFMSPYCQQPLQLGADIVVHSVTKYINGHGDVIGGIIVGKQEFIDQARFVGLKDITGGCMSPFNAWLTLRGVKTLGIRMERHCENALKIARFLEGHPSITRVYYPGLSSHPQYELGQRQMSLPGGIISFEIAGGLEAGRRMINSVELCLLAVSLGDTETLIQHPASMTHSPVAPEERLKAGITDGLIRLSVGLEDPEDIINDLEHAIRK-------

General information: TITO was launched using: RESULT: Template: 3MKJ.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 2157 -220680 -102.31 -591.64 target 2D structure prediction score : 0.58 Monomeric hydrophicity matching model chain A : 0.85 3D Compatibility (PKB) : -102.31 2D Compatibility (Sec. Struct. Predict.) : 0.58 1D Compatibility (Hydrophobicity) : 0.85 QMean score : 0.577

(partial model without unconserved sides chains): PDB file : Tito_3MKJ.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3MKJ-query.scw PDB file : Tito_Scwrl_3MKJ.pdb: