Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKKTMSAITAAAAVTSCFTGFGAASFSAPAKAAAQTNTLSENTNQSAAELVKNLYNTAYKGEMPQQAQGLTINKSTKGDVHAAFGEPERPVGGDNRF--DLY--HWNMGQPGYGFSYHK-DMTISEIRYFGTGVERQ---------LNLGGVTPEVLQKQLG-PVNRVLTVPFTDEIDYVYDTGRYELHFVIGTDQTADHVNLKAK 3GMV Chain:X ((12-107)) ----------------------------------------------------------------------QFG-MTFDEVWEIGGGEAACDTGGVIGDSILCFTESGDYAPYGGFSFTDEGE-LWSKRNEYLYKAKTPSVKLSHYNRTALGMTEAQLWAAVPKDSCVS--------------------------------------

General information: TITO was launched using: RESULT: Template: 3GMV.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain X - contact count / total energy / energy per contact / energy per residue : 210 -21963 -104.59 -271.15 target 2D structure prediction score : 0.58 Monomeric hydrophicity matching model chain X : 0.54 3D Compatibility (PKB) : -104.59 2D Compatibility (Sec. Struct. Predict.) : 0.58 1D Compatibility (Hydrophobicity) : 0.54 QMean score : 0.274

(partial model without unconserved sides chains): PDB file : Tito_3GMV.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3GMV-query.scw PDB file : Tito_Scwrl_3GMV.pdb: