Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKTCFLSIWRVVDPIYFFFSRLSLIDNDQKSVFRVRLTKYKGHHVVLSDGTHIRKNDVLVKIHLHNIKLIRELQSIESAVRKGIIIYQKVYQSMP------LLLDYINNHKKS--EKIKGIIGITMLDKGVERLGFDV------ITPVNPFYRCFKKVS--------HVPILYLTSKPVSLRHLPNSSYLFISKEKLQKTYQKKD 4BV4 Chain:R ((169-295)) ---------------------------------------------------------------------LENLESIEFGSNKLRQMPRGIFGKMPKLKQLNLWSNQLHNLTKHDFEGATSVLGIDIHDNGIEQLPHDVFAHLTNVTDINLSANLFRSLPQGLFDHNKHLNEVRLMNNRVPLATLP--SRLFANQPELQ-------

General information: TITO was launched using: RESULT: Template: 4BV4.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain R - contact count / total energy / energy per contact / energy per residue : 357 -37696 -105.59 -359.01 target 2D structure prediction score : 0.28 Monomeric hydrophicity matching model chain R : 0.65 3D Compatibility (PKB) : -105.59 2D Compatibility (Sec. Struct. Predict.) : 0.28 1D Compatibility (Hydrophobicity) : 0.65 QMean score : 0.225

(partial model without unconserved sides chains): PDB file : Tito_4BV4.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4BV4-query.scw PDB file : Tito_Scwrl_4BV4.pdb: