Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MGKVYIVGAGPGDPDLLTIKALKAIEKADVILYDRLVNKEILQYAKEQADLIYCGKLPDFHTMKQETINRFLVKYAQKGKMVVRLKGGDPFVFGRGGEEAECLSENGIPFEIIPGITSGIAAAAYAGIPVTHRDAGSNVAFVT-----GHYKKEEDFEEKWKALATGIDTLVIYMGIKNVQQIERKLLENGRDGSTPAAFIHWGTTDKQKSVFCTVDTLSETVIKENITNPSLIVIGNVVNYHYKLEWFESELKKQDLSEAL 1V9A Chain:A ((1-223)) -GRVYLVGAGPGDPELLTLKAYRLLKEAPVVLYDRLVDERVLALA--PGEKVYVGK-------KQEEIHRLLLRHARAHPFVVRLKGGDPMVFGRGGEEVLFLLRHGVPVEVVPGVTSLLA----SGLPLTHRGLAHGFAAVSGVLEGGGYPDLRPFAR--------VPTLVVLMGVGRRVWIAKELLRLGRDPREPTLFVERASTPKERRVHARLEEVAEG--KVEVRPPALWILGEVVR---------------------

General information: TITO was launched using: RESULT: Template: 1V9A.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1161 -143998 -124.03 -679.23 target 2D structure prediction score : 0.65 Monomeric hydrophicity matching model chain A : 0.79 3D Compatibility (PKB) : -124.03 2D Compatibility (Sec. Struct. Predict.) : 0.65 1D Compatibility (Hydrophobicity) : 0.79 QMean score : 0.625

(partial model without unconserved sides chains): PDB file : Tito_1V9A.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1V9A-query.scw PDB file : Tito_Scwrl_1V9A.pdb: