Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MHITTKRLLIREFEFKDWQAVYEYTSDSNVMKY-IPEGVFTEEDAKAFVNKNKGDNAEKFPVILRDEDCLIGHIVFYKY-FGEHTYEIGWVFNPNYQNKGYASEAAQAILEYGFKEMNLHRIIATCQPENIPSYRVMKKIGMRREGFFKKCIPKGNEWWDEYYYAILEEEWN 4MJ8 Chain:A ((5-146)) ----NSQLTLRALERGDLRFIHNLNNNRNIMSYWFEEPYESFDELEELYNKHIHDNAERRFVVEDAQKNLIGLVELIEINYIHRSAEFQIIIAPEHQGKGFARTLINRALDYSFTILNLHKIYLHVAVENPKAVHLYEECGFVEEG--------------------------

General information: TITO was launched using: RESULT: Template: 4MJ8.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 506 -84886 -167.76 -606.33 target 2D structure prediction score : 0.72 Monomeric hydrophicity matching model chain A : 0.73 3D Compatibility (PKB) : -167.76 2D Compatibility (Sec. Struct. Predict.) : 0.72 1D Compatibility (Hydrophobicity) : 0.73 QMean score : 0.626

(partial model without unconserved sides chains): PDB file : Tito_4MJ8.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4MJ8-query.scw PDB file : Tito_Scwrl_4MJ8.pdb: