Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKNTMKRMFCSMTVLVTAPYNEEGRKELENLFGSVAYQSWKEQGRAYREDELIQLLKATNATGLITELDQVTDSVFASVPELSFVGVCRGMPSNVDVAAASKRGIPVFYTPGRNAQAVAEMFIGNVISFLRHTSASNQWLKDGEWDSDYLQAYVKFKGNELTGKTVGMIGFGAVGQRIAKLLTAFDCKIKYYDPYIQDDHPL---YEKASLKTVFSDSDIVSVHLPRTEETLGLIDRQYFDLMKESAIFVNTSRAVVVNREDLLFVLKEHKISGAILDVFYHEPPEESDYELISLPNVLATPHLAGATFEVEDHHVTILNKALKKWKGEKTLNIQTMYNKDALKTGG 3DDN Chain:A ((35-316)) -------------------------------------------------DKLLAAVPEADAL-LVRSATTVDAEVLAAAPKLKIVARAGVGLDNVDVDAATARGVLVVNAPTSNIHSAAEHALALLLAASRQIPAADASLREHTWKRS------SFSGTEIFGKTVGVVGLGRIGQLVAQRIAAFGAYVVAYDPYVSPARAAQLGIELLSLDDLLARADFISVHLPKTPETAGLIDKEALAKTKPGVIIVNAARGGLVDEAALADAITGGHVRAAGLDVFATEPC--TDSPLFELAQVVVTPHLGASTAEAQDRAGTDVAESVR-------LALAGEFVPDAVNVGG

General information: TITO was launched using: RESULT: Template: 3DDN.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1436 -102259 -71.21 -366.52 target 2D structure prediction score : 0.59 Monomeric hydrophicity matching model chain A : 0.75 3D Compatibility (PKB) : -71.21 2D Compatibility (Sec. Struct. Predict.) : 0.59 1D Compatibility (Hydrophobicity) : 0.75 QMean score : 0.563

(partial model without unconserved sides chains): PDB file : Tito_3DDN.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3DDN-query.scw PDB file : Tito_Scwrl_3DDN.pdb: