Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSTIKVKSAHKDGQIKLEDLDVVCNKLCKRNNSVLFKLEKYLNKKLLSDPELTEIRDTILTVSGELSRLRDNLVTDGDSNEGLQ 3ZH2 Chain:A ((200-231)) -------------------------------------LQEFINNKLISDAELEAIFDRTVNTALEIVNL---------------

General information: TITO was launched using: RESULT: Template: 3ZH2.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 22 -6390 -290.43 -199.67 target 2D structure prediction score : 0.56 Monomeric hydrophicity matching model chain A : 0.69 3D Compatibility (PKB) : -290.43 2D Compatibility (Sec. Struct. Predict.) : 0.56 1D Compatibility (Hydrophobicity) : 0.69 QMean score : 0.500

(partial model without unconserved sides chains): PDB file : Tito_3ZH2.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3ZH2-query.scw PDB file : Tito_Scwrl_3ZH2.pdb: