Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSDKTMKDIQAEVDRYIGQFKEGYFSPLAMMARLTEELGELAREVNHRYGEKPKKATEDDK---SMEEEIGDVLFVLVCLANSLDISLEEAHDRVMHKFNTRDKDRWTRKEEGK 2Q4P Chain:A ((48-124)) ---------------------EQFHQPRNLLLALVGEVGELAELFQWKSDTEPGPQAWPPKERAALQEELSDVLIYLVALAARCHVDLPQA---VISKMDT-------------

General information: TITO was launched using: RESULT: Template: 2Q4P.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 163 -1919 -11.77 -25.93 target 2D structure prediction score : 0.77 Monomeric hydrophicity matching model chain A : 0.76 3D Compatibility (PKB) : -11.77 2D Compatibility (Sec. Struct. Predict.) : 0.77 1D Compatibility (Hydrophobicity) : 0.76 QMean score : 0.372

(partial model without unconserved sides chains): PDB file : Tito_2Q4P.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2Q4P-query.scw PDB file : Tito_Scwrl_2Q4P.pdb: