Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MLEKIIKQKKEEVKTLVLPVEQPFEKRSFKEALASPNRFIGLIAEVKKASPSKGLIKEDFVPVQIAKDYEAAKADAISVLTDTPFFQGENSYLSDVKRAVSIPVLRKDF-IDSLQVEESRRIGADAILLIGEVLDPLHLHELYLEAGEKGMDVLVEVHDASTLEQILKVFTPDILGVNNRNLKTFETSVKQTEQIASLVPKESLLVSESGIGSLEHLTFVNEHGARAVLIGESLMRQTSQRKAIHALFRE 1PII Chain:A ((33-249)) ---------------------QP-STRHFYDALQGAR--TAFILECKKASPSKGVIRDDFDPARIAAIYKH-YASAISVLTDEKYFQGSFNFLPIVSQIAPQPILCKDFIIDPYQIYLARYYQADACLLMLSVLDDDQYRQLAAVAHSLEMGVLTEVSNEEEQERAI-ALGAKVVGINNRDLRDLSIDLNRTRELAPKLGHNVTVISESGINTYAQVRELS-HFANGFLIGSALMAHDDLHAAV------

General information: TITO was launched using: RESULT: Template: 1PII.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1231 -61632 -50.07 -285.33 target 2D structure prediction score : 0.52 Monomeric hydrophicity matching model chain A : 0.76 3D Compatibility (PKB) : -50.07 2D Compatibility (Sec. Struct. Predict.) : 0.52 1D Compatibility (Hydrophobicity) : 0.76 QMean score : 0.338

(partial model without unconserved sides chains): PDB file : Tito_1PII.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1PII-query.scw PDB file : Tito_Scwrl_1PII.pdb: