Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNTFYDVQQLLKTFGHIVYFGDRELEIEFMLDELKELYMNHMIEKEQWARAAAVLRKELEQTKNGRDFYKG 2KRC Chain:A ((66-78)) --------------GRFLALSDQTWGL--------------------------------------------

General information: TITO was launched using: RESULT: Template: 2KRC.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 14 -2754 -196.71 -211.85 target 2D structure prediction score : 0.46 Monomeric hydrophicity matching model chain A : 0.61 3D Compatibility (PKB) : -196.71 2D Compatibility (Sec. Struct. Predict.) : 0.46 1D Compatibility (Hydrophobicity) : 0.61 QMean score : 0.564

(partial model without unconserved sides chains): PDB file : Tito_2KRC.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2KRC-query.scw PDB file : Tito_Scwrl_2KRC.pdb: