Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKIGLIGLGKMGINIGKQFIDRNHQVVGYDVNQAAVDELKAYGAEGTT----NLKEFISLLHPPRILWVMVPHGI-VDAVLRDVSPLLSKGDMIIEAGNSHYKESIRRYNQMKEAGIHYLDAGTSGGMEGARHGACFMVGGDHEAWEIVEPLFRDTAV-----ENGYLYAGEAGSGHFLKMIHNGIEYGMMAAIGEGFEVLENSQFDFDYEKVARV---WNHGSVIRSWLMGLTERAFAKDAK-----LDQIKGIMHSSGEGKWTVETALDLQTATPVIAMSLMMRYRSLTDDTFTGKVVAALRNEFGGHATEKK 2ZYA Chain:A ((17-306)) -QIGVVGMAVMGRNLALNIESRGYTVSIFNRSREKTEEVIAENPGKKLVPYYTVKEFVESLETPRRILLMVKAGAGTDAAIDSLKPYLDKGDIIIDGGNTFFQDTIRRNRELSAEGFNFIGTGVSGGEEGALKGPSIMPGGQKEAYELVAPILTKIAAVAEDGEPCVTYIGADGAGHYVKMVHNGIEYGDMQLIAEAYSLLKGG-LNLTNEELAQTFTEWNNGE-LSSYLIDITKDIFTKKDEDGNYLVDVILDEAANKGTGKWTSQSALDLGEPLSLITESVFARYISSLKD----------------------

General information: TITO was launched using: RESULT: Template: 2ZYA.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1283 6972 5.43 25.63 target 2D structure prediction score : 0.75 Monomeric hydrophicity matching model chain A : 0.77 3D Compatibility (PKB) : 5.43 2D Compatibility (Sec. Struct. Predict.) : 0.75 1D Compatibility (Hydrophobicity) : 0.77 QMean score : 0.468

(partial model without unconserved sides chains): PDB file : Tito_2ZYA.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2ZYA-query.scw PDB file : Tito_Scwrl_2ZYA.pdb: