Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MYDTIVIGAGQAGISIGYYLKQSDQKFIILDKSHEVGESWKDRYDSLVLFTSRMYSSLPGMHLEGEKHGFPSKNEIVAYLKKYVKKFEIPIQLRTEVISVLKI-KNYFLIKTNREEYQTKNLVIATGPFH-TPNIPSISKDLSDNINQLHSSQYKNSKQLAYGNVLVVGGGNSGAQIAVELSKE-RVTYLACSNKLVYFPLMIGKRSIFWWFDKLGVLHASHTSIVGKFIQKKGDPVFGHELKHAIKQKEIILKKRVIAAKQNEIIFKDSSTLEVNNIIWATGFRNPLCWINIKG-VLDQEGRIIHH-RGVSPVEGLYFIGLPWQHKRGSALLQGVGNDAEYIVKQMNGE 3LZW Chain:A ((7-312)) VYDITIIGGGPVGLFTAFYGGMRQASVKIIESLPQLGGQLSALYPEKYIYD------VAGFPK-------IRAQELINNLKEQMAKFDQTICLEQAVESVEKQADGVFKLVTNEETHYSKTVIITAGNGAFKPRKLELENAEQYEGKNLHYF-VDDLQKFAGRRVAILGGGDSAVDWALMLEPIAKEVSIIHRRD-KFRAHEH----SVENLHASK-------------VN----VLTPFVPA-ELIGE-----DKIEQLVLEEVKGDRKEILEIDDLIVNYGFVSSLGPIKNWGLDIE-KNSIVVKSTMETNIEGFFAAGDICTYEGKVNLIASGFGEAPTAVNNAKA-

General information: TITO was launched using: RESULT: Template: 3LZW.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1714 -32088 -18.72 -106.60 target 2D structure prediction score : 0.49 Monomeric hydrophicity matching model chain A : 0.67 3D Compatibility (PKB) : -18.72 2D Compatibility (Sec. Struct. Predict.) : 0.49 1D Compatibility (Hydrophobicity) : 0.67 QMean score : 0.391

(partial model without unconserved sides chains): PDB file : Tito_3LZW.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3LZW-query.scw PDB file : Tito_Scwrl_3LZW.pdb: