Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MFGIGARDLGIDLGTANTLVFVKGKGIVVREPSVVALQT--DTKSIVAVGNDAKNMIGRTPGNVVALRPMKDGVIADYETTATMMKYYINQAIKNKGMFARKPYVMVCVPSGITAVEERAVIDATRQAGARDAYPIEEPFAAAIGANLPVWEPTGSMVVDIGGGTTEVAIISLGGIVTSQSIRVAGDEMDDAIINYIRKTYNLMIGDRTAEAIKMEIGSAEAPEESDNM--EIRGRDLLTGLPKTIEITGKEISNALRDTVSTIVEAVKSTLEKTPPELAADIMDRGIVLTGGGALLRNLDKVISEETKMPVLIAEDPLDCVAIGTGKALEHIHLFKGKTR 4CZL Chain:A ((3-333)) -----SNDIAIDLGTANTLIYQKGKGIVLNEPSVVALRNVGGRKVVHAVGIEAKQML------MEAIRPMRDGVIADFEVAEEMIKYFIRKVHNRKG--SGNPKVIVCVPSGATAVERRAINDSCLNAGARRVGLIDEPMAAAIGAGLPIHEPTGSMVVDIGGGTTEVAVLSLSGIVYSRSVRVGGDKMDEAIISYMRRHHNLLIGETTAERIKKEIGTARAPADGEGLSIDVKGRDLMQGVPREVRISEKQAADALAEPVGQIVEAVKVALEATPPELADDIADKGIMLTGGGALLRGLDAEIRDHTGLPVTVADDPLSCVALGCGKVLEHPKWMKG---

General information: TITO was launched using: RESULT: Template: 4CZL.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1866 1776 0.95 5.53 target 2D structure prediction score : 0.64 Monomeric hydrophicity matching model chain A : 0.87 3D Compatibility (PKB) : 0.95 2D Compatibility (Sec. Struct. Predict.) : 0.64 1D Compatibility (Hydrophobicity) : 0.87 QMean score : 0.559

(partial model without unconserved sides chains): PDB file : Tito_4CZL.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4CZL-query.scw PDB file : Tito_Scwrl_4CZL.pdb: