Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MEVKEQLKLKELLFIMKQMPKTFKLIFTLERSLFLKLIRFSIITGILPIVSLYISQELINSLVTIRKEVSIVI--T---IFLTYLGVSFFSELISQISEFYNGKFQLNIGYKLNYKVMKKSSNLALKDFENPEIYDKLERVTKEISYKPYQII-QAIITMTTSFVTLLSSIA-FLMSWNPKVSLLLLVIPVISLFYFLKIGQEEFFIHWKRAGKERKSWYISYILTHDFSFKELKLYNLKDYLLNKYWDIKKSFIEQDTKILRKKTLLNLIYEIAVQLVGAVIIFIAIMSAFAGKIMVGNVMSYIRSVSLVQNHSQSIMTSIYSIYNSNLYMNQLYEFLELKEEKSQGHK-KPI-VEPIHSVVFQNVSFIYPNQGEQTLKHINVSLHKGERVAIVGPNGSGKKTFIKLLTGLYEVHEGDILINGINIKELDMDSYMNQIAALFQDFMKYEMTLKENIGFGQIDKLHQTNKMHEVLDIVRADFLKSHSSYQFDTQLGLWFDEGRQLSGGQWQKIALARAYFREASLYILDEPSSALDPIAEKETFDTFFSLS--KDKIGIFISHRLVAAKLADRIIVMDKGEIVGIGTHEELLKTC-PLYKKMDESENYMNPLEEEGSKWKEALYQG 4MYC Chain:A ((111-683)) ---------------------------------VLIALGLLISAKILNVQVPFFFKQTIDSMNIAWDDPTVALPAAIGLTILCYGVARFGSVLFGELRNAVFAKVAQNAIRTVSLQTFQHLMKLDLGWHLSRQTGGLTRAMDRGTKG-ISQVLTAMVFHIIPISFEISVVCGILTYQFGASFAAITFSTMLLYSIFTIKTTAWRTHFRR--DANKADNKAASVALDSLINFEAVKYFNNEKYLADKYNGSLMNYRDSQIKVSQSLAFLNSGQNLIFTTALTAMMYMGCTGVIGGNLTVGDLVLINQLVFQLSVPLNFLGSVYRDLKQSLIDMETLFKLRKNEVKIKNAERPLMLPENVPYDITFENVTFGYHP-DRKILKNASFTIPAGWKTAIVGSSGSGKSTILKLVFRFYDPESGRILINGRDIKEYDIDALRKVIGVVPQDTPLFNDTIWENVKFGRIDA--TDEEVITVVEKAQLAPLIKKLPQGFDTIVG---ERGLMISGGEKQRLAIARVLLKNARIMFFDEATSALDTHTEQALLRTIRDNFTSGSRTSVYIAHRLRTIADADKIIVLDNGRVREEGKHLELLAMPGSLYRELWTIQEDLDHLENE-----------

General information: TITO was launched using: RESULT: Template: 4MYC.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 2282 -18669 -8.18 -33.28 target 2D structure prediction score : 0.60 Monomeric hydrophicity matching model chain A : 0.71 3D Compatibility (PKB) : -8.18 2D Compatibility (Sec. Struct. Predict.) : 0.60 1D Compatibility (Hydrophobicity) : 0.71 QMean score : 0.363

(partial model without unconserved sides chains): PDB file : Tito_4MYC.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4MYC-query.scw PDB file : Tito_Scwrl_4MYC.pdb: