Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MRKINFFDTTLRDGEQSPGVNLNTQEKLAIAKQLERLGADIIEAGFPASSRGDFLAVQEIARTIKNCSVTGLARCVKGDIDAAWEALKDGAQPRIHVFIATSDIHLKHKLKMTREQVIERAVGMVKYAKERFPIVQ--WSAEDACRTELPFLAEIVEKVIDAGASVINLPDTVGYLAPAEYGNIFKYMKENV-PNIHKAKLSAHCHDDLGMAVANSLAAIENGADQIECAVNGIGERAGNAALEEIAVALHTRKDFYQVETGITLNEIKRTSDLVSKLTGMAVPRNKAVVGDNAFAHESGIHQDGFLKEKSTYEIISPELVGVTADALVLGKHSGRHAFKDRLTALGFQFDSEEINKFFTMFKELTEKKKEITDEDLVSLILEEKVTDRKIGYEFLSLQVHYGTSQVPTATLSLKNQENAQLIQEAATGAGSVEAIYNTLERCIDKDVELLDYRIQSNRKGEDAFAQVYVRVLVNGKESAGRGIAQDVLEASAKAYLNAVNRQLVFQSNMSGLKNHTAVGS 2ZYF Chain:A ((1-320)) MREWKIIDSTLREGEQFEKANFSTQDKVEIAKALDEFGIEYIEVTTPVASPQSRKDAEVLASLGLKAKVVTHIQC---RLDAAKVAVETGVQGIDLLFGTSK------GRDIPR--IIEEAKEVIAYIREAAPHVEVRFSAEDTFRSEEQDLLAVYEAVAPY-VDRVGLADTVGVATPRQVYALVREVRRVVGPRV---DIEFHGHNDTGCAIANAYEAIEAGATHVDTTILGIGERNGITPLGGFLARMYTLQPEY-VRRKYKLEMLPELDRMVARMVGVEIPFNNYITGETAFSHKAGMHLKAIYINPEAYEPYPPEVFGVKRKLIIA-----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 2ZYF.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1777 -22102 -12.44 -71.07 target 2D structure prediction score : 0.65 Monomeric hydrophicity matching model chain A : 0.69 3D Compatibility (PKB) : -12.44 2D Compatibility (Sec. Struct. Predict.) : 0.65 1D Compatibility (Hydrophobicity) : 0.69 QMean score : 0.457

(partial model without unconserved sides chains): PDB file : Tito_2ZYF.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2ZYF-query.scw PDB file : Tito_Scwrl_2ZYF.pdb: