Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKSKFEASIDNLKEIEMNAYAYELIREIVLPDMLGQDYSSMMYWAGKHLARKFPLESWEEFPAFFEEAGWGTLTNVSAKKQELEFE--------LEGPIISNRLK--------HQKEPCFQLEAGFIAEQIQLMNDQIAESYEQVKK--RADKVVLTVKWDMKDPV 4U9B Chain:A ((54-180)) -----------------------------VAQRLNMPIQDVVKAFGQFLFNGLA-SRHTDV---VD-KFDDFTSLVMGIHDVIHLEVNKLYHEPSLPHINGQLLPNNQIALRYSSPRRLCFCAEGLLFGAAQH-FQQKIQISHDTCMHTGADHCMLIIELQN----

General information: TITO was launched using: RESULT: Template: 4U9B.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 369 3787 10.26 34.74 target 2D structure prediction score : 0.51 Monomeric hydrophicity matching model chain A : 0.60 3D Compatibility (PKB) : 10.26 2D Compatibility (Sec. Struct. Predict.) : 0.51 1D Compatibility (Hydrophobicity) : 0.60 QMean score : 0.385

(partial model without unconserved sides chains): PDB file : Tito_4U9B.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4U9B-query.scw PDB file : Tito_Scwrl_4U9B.pdb: