Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MPLISIDSRKHLFYEEYGQGIPIIFIHPPGMGRKVFYYQRL----LSKHFRVIFPDLSGHGDSDHIDQPASISY----YANEIAQFMDALHIDKAVLFGYSAGGLIAQHIGFTRPDKVSHLILSGAYPAVHNVIGQKLHKLGMYLLEKNPGLLMKILAGSHTKDRQLRSILTDHMKKADQAHWHQYYLDSLGYNCIEQLPRLEMPMLFMYGGLRDWTFTNAGYYRRSCRHAEFFRLEYQGHQLPTKQWKTCNELVTGFVLTHHS 1UK9 Chain:A ((19-128)) -------------YHDVGEGQPVILIHGSGPGVSAYANWRLTIPALSKFYRVIAPDMVGFG---FTDRPENYNYSKDSWVDHIIGIMDALEIEKAHIVGNAFGGGLAIATALRYSERVDRMVLMGA------------------------------------------------------------------------------------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 1UK9.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 454 -27642 -60.88 -271.00 target 2D structure prediction score : 0.55 Monomeric hydrophicity matching model chain A : 0.62 3D Compatibility (PKB) : -60.88 2D Compatibility (Sec. Struct. Predict.) : 0.55 1D Compatibility (Hydrophobicity) : 0.62 QMean score : 0.516

(partial model without unconserved sides chains): PDB file : Tito_1UK9.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1UK9-query.scw PDB file : Tito_Scwrl_1UK9.pdb: