Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MIGVIDYGMGNLYSVSKALERVGVPYFVSEKPEE--LKEADAFILPGVGSFGDAMDNLGYTKLDQLIHDMVSEGRLLLGICLGMQLLFEESEENGTASGLGLLKGKAVRLKAEDEKGNKLKVPHMGWNRLSFHNESPLLTKTEQGYAYFVHSYYI-------DGMEENAL-LASADYGV-RVPAVVGKRNVFGAQFHPEKSSTVGMSILTQFTKMAAEQKVKK 1OX4 Chain:A ((6-235)) VVHVIDVESGNLQSLTNAIEHLGYEVQLVKSPKDFNISGTSRLILPGVGNYGHFVDNLFNRGFEKPIREYIESGKPIMGICVGLQALFAGSVESPKSTGLNYIDFKLSRFDDSEK-----PVPEIGWNSC-IPSENLFFGLDPYKRYYFVHSFAAILNSEKKKNLENDGWKIAKAKYGSEEFIAAVNKNNIFATQFHPEKSGKAGLNVIENFLKQQS------

General information: TITO was launched using: RESULT: Template: 1OX4.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1049 -25805 -24.60 -129.03 target 2D structure prediction score : 0.70 Monomeric hydrophicity matching model chain A : 0.81 3D Compatibility (PKB) : -24.60 2D Compatibility (Sec. Struct. Predict.) : 0.70 1D Compatibility (Hydrophobicity) : 0.81 QMean score : 0.515

(partial model without unconserved sides chains): PDB file : Tito_1OX4.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1OX4-query.scw PDB file : Tito_Scwrl_1OX4.pdb: