Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNIAVVTDSTAYIPKEMREQHQIHMIPLQVVFREETYREEI---ELDWKSFYEEVKKHNELPTTSQPPIGELVALYEELGKSYDAVISIHLSSGISGTFSSAAAADSMVDNIDVYP------FDSEISCLAQGFYALKAAELIKNGASSPEDIIKEL-EEMKKTVRAYFMVDDLAHLQRGGRLSSAQAFIGSLLKVKPILHFD-NKVIVPFEKIRTRKKAISRIYELLDEDASKGLPMRAAVIHANREEEAAKIIEELSAKYPHVEFYNSYFGAVIGTHLGEGALGICWCFK 3PL5 Chain:A ((36-312)) -TFKILTDSTADLPESWTQENDVQVLGLTVQLDGITY-ETVGPDRLTSRVLLEKIAAGSK-PTTSQVNVGQFESYFRQSAENGQEVLYIAFSSVLSGTYQSAVMARDIV--LEEYPQASIEIVDTLAATGGEGYLAMLAAQAREEGKSLKE--TKELILDVGPRLRTFFLVDNLYHLMRGGRLS--------LVNIKPLLWLDASGKLVPIAKLRGRKKGMKEMLKRATADVAHD---TAVVAYANDSEAAENLKEQLLANEKIKNVVTLPLGPVISTHVGPNTLAV-----

General information: TITO was launched using: RESULT: Template: 3PL5.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1328 -20225 -15.23 -78.39 target 2D structure prediction score : 0.61 Monomeric hydrophicity matching model chain A : 0.77 3D Compatibility (PKB) : -15.23 2D Compatibility (Sec. Struct. Predict.) : 0.61 1D Compatibility (Hydrophobicity) : 0.77 QMean score : 0.450

(partial model without unconserved sides chains): PDB file : Tito_3PL5.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3PL5-query.scw PDB file : Tito_Scwrl_3PL5.pdb: