Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence --------------------------MKLFGMIFLIATVAFILLGVLLKLAAFFFVSILTLIAAIVLFTVLKKNQHNQT------------------------------ 4G6V Chain:B ((2-104)) AIDLFCYLSIDRGAAESDLNKIRSNHSELFEGKFLISPVRDADFSLKEIAAEH------GLVAESFFLVSLNDKNSADLIPIVSKILVDGFNGGAILILQDNEYRRTSL

General information: TITO was launched using: RESULT: Template: 4G6V.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 74 -11487 -155.23 -244.40 target 2D structure prediction score : 0.40 Monomeric hydrophicity matching model chain B : 0.56 3D Compatibility (PKB) : -155.23 2D Compatibility (Sec. Struct. Predict.) : 0.40 1D Compatibility (Hydrophobicity) : 0.56 QMean score : 0.518

(partial model without unconserved sides chains): PDB file : Tito_4G6V.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4G6V-query.scw PDB file : Tito_Scwrl_4G6V.pdb: