Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTAHDQELRRRAYEEVEKKEPIANSDPHRQHFHIMPPVGLLNDPNGVIYWKGSYHVFFQWQPFQTGHG-AKFWGHYTTQDVVNWKREEIALAPSDWFDKNGCYSGSAVTKDDRL-YLFYTGNVRDQDGNRETYQCLA----VSDDGLS---FEKKGVVARLPEGYTAH-FRDPK-VW-EHEGTWYMVIGAQTENLKGQAVLFASDNLTEW-RFLGPITGAGFNGLDDFGYMWECPDLF-----SLQGSDVLIV--SPQGLEADGFRYQNVYQSG-YFVGRLDYNKPELKHGEFTELDQGFD---FYAPQTLEDD-QGRRILFAWMAVPDQDEGSHPTIDCHWIHCMTLPRQLTL--SGQKLIQQPLPELKAMRRNEKKIHINMHGSSGALPVEKPERTEILLEDIHTESGFSISIRGTATFSFHKDEGIVTLERKSFDGKRTEARHCRIKDLHTVHMFLDASSVEIFINNGEEVLSARYFPFPGNHEVTASATGKSEMNVGIWTLM 1ST8 Chain:A ((6-345)) --------------------------PYRTGYHFQPPSNWMNDPNGPMLYQGVYHFFYQYNPYAATFGDVIIWGHAVSYDLVNWIHLDPAIYPTQEADSKSCWSGSATILPGNIPAMLYTGS----DSKSRQVQDLAWPKNLSDPFLREWVKHPKNPLITPPEGVKDDCFRDPSTAWLGPDGVWRIVVGGDRDN-NGMAFLYQSTDFVNWKRYDQPLSSADATG------TWECPDFYPVPLNSTNGLDTSVYGGSVRHVMKAGFEGHDWYTIGTYSPDRENFLPQNGLSLTGSTLDLRYDYGQFYASKSFFDDAKNRRVLWAW--VPETDSQAD-DIEKGWAGLQSFPRALWIDRNGKQLIQWPVEEIEELRQNQVNLQ------------------------------------------------------------------------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 1ST8.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1869 15563 8.33 49.72 target 2D structure prediction score : 0.59 Monomeric hydrophicity matching model chain A : 0.70 3D Compatibility (PKB) : 8.33 2D Compatibility (Sec. Struct. Predict.) : 0.59 1D Compatibility (Hydrophobicity) : 0.70 QMean score : 0.355

(partial model without unconserved sides chains): PDB file : Tito_1ST8.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1ST8-query.scw PDB file : Tito_Scwrl_1ST8.pdb: