Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSDEQKKPEQIHRRDILKWGAMAGAAVAIGASGLGGLAPLVQTAAKPSKKDEKEEEQIVPFYGKHQAGITTAHQTYVYFAALDV---------TAKDKSDIITLFRNWTSLTQMLTSGKKMSAEQRNQYLPPQDTGE---SADLSPSNLTVTFGFGPGFFEK-DGKDRFGLKSKKPKHLAALPAMPNDNLDEKQGGGDICIQVCADDEQVAFHALRNLLNQAVGTCEVRFVNKGFLSGGKNGE----TPRNLFGFKDGTGNQSTKDDTLMNSIVWIQSGEPD--WMTGGTYMAFRKIKMFLEVWDRSSLKDQEDTFGRRKSSGAPFGQKKETDPVKLNQ-IPSNSHVSLA---KSTGKQILRRAFSYTEGLDPKTGYMDAGLLFISFQKNPDNQFIPMLKALSAK-DALNEYTQTIGSALYACPGGCKKGEYIAQRLLES 5FW4 Chain:A ((71-429)) -----------------------------------------------------------------QPGISGPAPAFVHVIALDLAEEARKNPDTARDSA--AAALRSWTELAARLHEES------------PHDIAEGAASAGLLPASLMVTVGIGGSLLSAIDAEDR------RPDALADLPEFSTDDLHPRWCGGDFMLQVGAEDPMVLTAAVEELVAAAADATAVRWSLRGFRRTAAAARDPDATPRNLMGQIDGTANPAQDHPLFDRTITARPADNPAHAWMDGGSYLVVRRIRMLLTEWRKLDVAARERVIGRRLDTGAPLGSRNET-------LIPENAHVRLASPENNLGARMFRRGYSYDQGWR-DDGVRDAGLLFMAWQGDPATGFIPVQRSLADQGDALNRYIRHEGSALFAVP-AAREGRYLGQDLIE-

General information: TITO was launched using: RESULT: Template: 5FW4.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1637 40787 24.92 127.06 target 2D structure prediction score : 0.69 Monomeric hydrophicity matching model chain A : 0.77 3D Compatibility (PKB) : 24.92 2D Compatibility (Sec. Struct. Predict.) : 0.69 1D Compatibility (Hydrophobicity) : 0.77 QMean score : 0.390

(partial model without unconserved sides chains): PDB file : Tito_5FW4.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5FW4-query.scw PDB file : Tito_Scwrl_5FW4.pdb: