Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKKEEWILPYIKQNARLFVLVIFLGAVTIFSAAFLMFTSGFLISKAATRPENILLIYVPIVAVRTFGIARSVSRYVERLVGHHIILKIVSDMRVRLYNMLEPGALMLRSRFRTGDMLGILSEDIEHLQDAFLKTIFPAISALLLYAVSVIALGFFSWPFAILLALYLFVLVVLFPVVSLLVTRAKNAKLKSGRNVLYSRL---TDAVMGVSDWMFSGRRHAFIDAYEKEERDWFELERKKQRFTRWRDFAAQCLVAGLILLMLFWTAGQQA----------DGELAKTMIAAFVLVVFPLTEAFLPLSDALGEVPGYQDSIRRMNNVAPQPEASQTESGDQILDLQDV--TLAFRDVTFSY-DNSSQVLHNFSFTLRQGEKMALLGRSGSGKSTSLALIEGALKPDSGSVTLN--GVETALLKDQIADAVAVLNQKPHLFDTSILNNIRLGNGEASDEDVRRAAKQVKLHDYIESLPDGYHTSVQETGIRFSGGERQRIALARILLQDTPIIILDEPTVGLDPITERELMETVFEVLKGKTILWITHHLAGVEAADKIVFLENGKTEMEGTHEELLAANERYRRLYHLDVPVK 4Q4J Chain:A ((86-566)) -----------------------------------------------------------------------------------------ADLRRDLFRKVLSFSISNVNRFHTSSLITRLTNDVTQLQNLVMMLLRIVVRAPLLF------VGGIVMAVSINVKLSSVLIFLIPPIVLLFVWLTKKG------NPLFRKIQESTDEVNRVVRENLLGVR--VVRAFRREEYENENFRKANESLRRSIISAFSLIVFALPLFIFIVNMGMIAVLWFGGVLVRNNQMEIGSIMAYTNYLMQIMFSLMMIGNILNFIVRASASAKRVLEVLNEKPA--IEEADNALALPNVEGSVSFENVEFRYFENTDPVLSGVNFSVKPGSLVAVLGETGSGKSTLMNLIPRLIDPERGRVEVDELDVRTVKLKD-LRGHISAVPQETVLFSGTIKENLKWGREDATDDEIVEAAKIAQIHDFIISLPEGYDSRVERGGRNFSGGQKQRLSIARALVKKPKVLILDDSTCSVDPITEKRILDGLKRYTKGSTTFIITQKIPTALLADKILVLHEGKVAGFGTHKELLEHSKPYREIYE------

General information: TITO was launched using: RESULT: Template: 4Q4J.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1915 -11622 -6.07 -25.10 target 2D structure prediction score : 0.68 Monomeric hydrophicity matching model chain A : 0.72 3D Compatibility (PKB) : -6.07 2D Compatibility (Sec. Struct. Predict.) : 0.68 1D Compatibility (Hydrophobicity) : 0.72 QMean score : 0.454

(partial model without unconserved sides chains): PDB file : Tito_4Q4J.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4Q4J-query.scw PDB file : Tito_Scwrl_4Q4J.pdb: