Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MIMKMTHLNMKDFNKPNEPFVVFGRMIPAFENGVWTYTEERFSKPYFKQYEDDDMDVSYVEEEGKAAFLYYLENNCIGRIKIRSNWNGYALIED------------IAVAKDYRKKGVGTALLHKAIEWAKENHFC-GLMLETQDINISACHFYAKHHFIIGAVDTMLYSNFPTANEIAIFWYYKF 2X7B Chain:A ((44-145)) --------------------------------------------------------VEHLKEYGLAFFVAIVDNSVVGYIMPRIEW-GFSNIKQLPSLVRKGHVVSIAVLEEYRRKGIATTLLEASMKSMKNDYNAEEIYLEVRVSNYPAIALYEKLNF---------------------------

General information: TITO was launched using: RESULT: Template: 2X7B.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 331 -13008 -39.30 -146.15 target 2D structure prediction score : 0.58 Monomeric hydrophicity matching model chain A : 0.62 3D Compatibility (PKB) : -39.30 2D Compatibility (Sec. Struct. Predict.) : 0.58 1D Compatibility (Hydrophobicity) : 0.62 QMean score : 0.375

(partial model without unconserved sides chains): PDB file : Tito_2X7B.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2X7B-query.scw PDB file : Tito_Scwrl_2X7B.pdb: