Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MVNKDVTKVCIGKIEELPEQLGKTVYI-EDKELAVFKLSDGSIRAIENRCPHKGGVLAEGIVSGQYVFCPMHDWKISLEDGIVQEPDHGCVKTYETLIEGEHVYLVY 1VM9 Chain:A ((1-100)) ------SFEKICSLDDIWVGEMETFETSDGTEVLIVNSEEHGVKAYQAMCPHQEILLSEGSYEGGVITCRAHLWTFNDGTGHGINPDDAALAEYPVEVKGDDIYVS-

General information: TITO was launched using: RESULT: Template: 1VM9.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 476 -64878 -136.30 -655.33 target 2D structure prediction score : 0.40 Monomeric hydrophicity matching model chain A : 0.71 3D Compatibility (PKB) : -136.30 2D Compatibility (Sec. Struct. Predict.) : 0.40 1D Compatibility (Hydrophobicity) : 0.71 QMean score : 0.550

(partial model without unconserved sides chains): PDB file : Tito_1VM9.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1VM9-query.scw PDB file : Tito_Scwrl_1VM9.pdb: