Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSHSQEKIALITGASSQGDIGTAICRKLASQGIHIFFTHWNSDTAWIEEFQQEILRMGVRCEAMKIDLSDAHAAFTIHEKISDKLGYPSILINNAAHSASDNYVSLDAKSLDEHYAVNMRSNFLLCVEFARRFKKSNLISGRIINMTSGQDLGPL--PGELAYAATKGAISAFTRSLSQELAPLGITVNAVNPGPTDSTWMTDEI----RNFLSPKFPMGRIGTPDDAARMIAFLASDEAEWITGQIIHSEGGFIRG 4BO0 Chain:B ((26-266)) ----QGKVALVTGASR--GIGQAIALELGRLGAVVIGTATSASGA--EKIAETLKANGVEGAGLVLDVSSDESVAATLEHIQQHLGQPLIVVNNAGI-----------DEWFDVVNTNLNSLYRLSKAVLRGMTKARW--GRIINI--GSVVGAMGNAGQTNYAAAKAGLEGFTRALAREVGSRAITVNAVAPGFID-TDMTRELPEAQREALLGQIPLGRLGQAEEIAKVVGFLASDGAAYVTGATVPVNGGM---

General information: TITO was launched using: RESULT: Template: 4BO0.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 1216 -149535 -122.97 -667.56 target 2D structure prediction score : 0.73 Monomeric hydrophicity matching model chain B : 0.78 3D Compatibility (PKB) : -122.97 2D Compatibility (Sec. Struct. Predict.) : 0.73 1D Compatibility (Hydrophobicity) : 0.78 QMean score : 0.588

(partial model without unconserved sides chains): PDB file : Tito_4BO0.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4BO0-query.scw PDB file : Tito_Scwrl_4BO0.pdb: