Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence -------------------------MKKTLALAATAAVLMLSACSSGFGGEKEEEITQKTAKSSEKAIVPKYNISDSY---------------YKMVLPFKAGKARGLTTEQLNTRLDIDEFETGLMRLAQDSFSTDDYLFQEGQY-------------LDEDTVLSW---------LARKKTGSDLKKAEKEDKNFKNEGLNPALPSSGSTEEKN--ESSPIYLASMLEHDYLVRKDKNSIQLGGVMIGL---------------ALNSVYYYREKTGDPQKEVEIKDSTLRQQGEKIAQEVINRLRKKDNLKNVPITVALYKQASKTSIVP---GNFIAKTEVKAGSTDISNWDDINE-----------------------KYV---------FYPADTTTAEKY-----PDDTEVFKRFKN-SIEEYFPNYTGVVGT------ALYENDEMKKMKIDI-PMQFYGKSEVVAFTQFLTGEVMDYYSK---SSVDVEVN-----------ITSSDGQEAVIIRNAGDKEPTVHIYD-- 5CWW Chain:B ((3-593)) KIKSFAPAWLNEPAPGHKLFAPKPGPRRTIARRGTEIFVACGK-QIRWGDLAQLKESWESRPSDDGAATAGYRIIKTPVADDIRQLVMSPNQDFLAVLTSHTVHICILPDSSHLHIQDTTPFKPKFWTLGPTTHVTSRSAVVSAVWHPLGVNGHALVTVTEDAIVRVWELSTADRWTFDAPTLAIDLKKLADATYLDQDFGVSTSATNKGFSPDAFDMEVAAACFPTRDSGGWAPMTLWLAMTSGDVYALCPLLPQRWTPPPTLIPSLSASIVAKVAAAEDNPESTPEERLVAQQQLEWMSEIDNQEPKLVEEATGEATIEVYTRPSRPGLVPKLQGPFDFDLNPEDEQDDEVELKDIYVIGEKPRNGLSLNIICLLSTSGQVKICLDIDGVEAQWLPPRSKNKRLFAPPPEPPSLLTFQTFDTLKPAEVTPDGWPMFSEDATSPYSFYVTHPAGITYISLTPWVFRLESELQSDSEAGTEFRIDLLAKGQGSERDRIFTQTRTQSPLAAATSIDDPDLGYFILSATQTDPIALFFETP

General information: TITO was launched using: RESULT: Template: 5CWW.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 1542 -35743 -23.18 -90.49 target 2D structure prediction score : 0.50 Monomeric hydrophicity matching model chain B : 0.63 3D Compatibility (PKB) : -23.18 2D Compatibility (Sec. Struct. Predict.) : 0.50 1D Compatibility (Hydrophobicity) : 0.63 QMean score : 0.107

(partial model without unconserved sides chains): PDB file : Tito_5CWW.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5CWW-query.scw PDB file : Tito_Scwrl_5CWW.pdb: