Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MEGYLVLEDGTSFSGELDGHENCTGEAVFFTGMTGYQEVLTDPSYKGQIIVFTYPLIGNYGINEKDFESKKPQVKAAVVYEACDHFSHYEAVYSLKEYLQKWNIPLLTHVDTRAVVKKIRANGTMGATVTASKEGAEIALQPENVAEQASAQEISTFGDGNKHIALIDFGYK--KSIASSLVKRGCKVTVVPYQQMEAVY-NIKPDGIVLSNGPGDPKAIQPYLGKIKSI-ISRFPTLGICLGHQLIALAFGGNTFKLPFGHRGANHPVIDRK--------TK-------RVFMTSQNHSYVVDEQSINEEELTIRFHHVNDTSVEGLAHKKLPVMSVQFHPEAHPGPAESEWIFDDYLKNVIPARREIAHA 3TQI Chain:A ((7-246)) ----------------------------------------------------------------------------------------------------------------------------------------------------------------QHRILILDFGSQYAQLIARRVREIGVYCELMPCDIDEETIRDFNPHGIILSGGPE----------APAFIFEIGCPVLGICYGMQTMAYQLGGKVN-----EFGHA-QLRVLNPAFLFDGIEDQVSPQGEPLLDVWMSHGDIVSE--LPP-GFEATACTD-NSPLAAMADFKRRFFGLQFHPEVTHTP-QGHRILAHFVIHICQCI------

General information: TITO was launched using: RESULT: Template: 3TQI.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 954 -128925 -135.14 -776.66 target 2D structure prediction score : 0.52 Monomeric hydrophicity matching model chain A : 0.60 3D Compatibility (PKB) : -135.14 2D Compatibility (Sec. Struct. Predict.) : 0.52 1D Compatibility (Hydrophobicity) : 0.60 QMean score : 0.420

(partial model without unconserved sides chains): PDB file : Tito_3TQI.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3TQI-query.scw PDB file : Tito_Scwrl_3TQI.pdb: