Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MVNKDVTKVCIGKIEELPEQLGKTVYIEDKELAVFKLSDGSIRAIENRCPHKGGVLAEGIVSGQYVFCPMHDWKISLEDGIVQE-PDHGCVKTYETLIEGEHVYLVY 3DQY Chain:A ((1-99)) ------TWTYILRQGDLPPGEMQRYEGGPEPVMVCN-VDGEFFAVQDTCTHGDWALSDGYLDGDIVECTLHFGKFCVRTGKVKALPACKPIKVFPIKVEGDEVHVD-

General information: TITO was launched using: RESULT: Template: 3DQY.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 486 -63912 -131.51 -652.16 target 2D structure prediction score : 0.51 Monomeric hydrophicity matching model chain A : 0.70 3D Compatibility (PKB) : -131.51 2D Compatibility (Sec. Struct. Predict.) : 0.51 1D Compatibility (Hydrophobicity) : 0.70 QMean score : 0.564

(partial model without unconserved sides chains): PDB file : Tito_3DQY.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3DQY-query.scw PDB file : Tito_Scwrl_3DQY.pdb: