Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MGKGDVLEAAPTTTAYHSLMDEYGYEVGKAIGHGSYGSVYEAFYTKQKVMVAVKIISKKKASDDYLNKFLPREIQVMKVLRHKYLINFYRAIESTSRVYIILELAQGGDVLEWIQRYGACSEPLAGKWFSQLTLGIAYLHSKSIVHRDLKLENLLLDKWENVKISDFGFAKMVPSNQPVGCSPSYRQVNCFSHLSQTYCGSFAYACPEILRGLPYNPFLSDTWSMGVILYTLVVAHLPFD---DTNLKKLLRETQKEVTFPANHTISQECKNLILQMLRQATKRATILDIIKDSWVLKFQPEQPTHEIRLLEAMCQLHNTTKQHQSLQITT 2X8D Chain:A ((9-264)) ------------------------WDLVQTLGEGAYGEVQLAVNRVTEEAVAVKIVDMKRAVDCPEN--IKKEICINKMLNHENVVKFYGHRREGNIQYLFLEYCSGGELFDRIEPDIGMPEPDAQRFFHQLMAGVVYLHGIGITHRDIKPENLLLDERDNLKISDFGLATVF-------------RYNNRERLLNKMCGTLPYVAPELLKRREFHAEPVDVWSCGIVLTAMLAGELPWDQPSDSCQEYSDWKEKKTYLNPWKKIDSAPLALLHKILVENPSARITIPDIKKDRWYNKPLKKGAKRPRVTSG-------------------

General information: TITO was launched using: RESULT: Template: 2X8D.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output 3D Compatibility (PKB) -131266 for 1958 contacts (-67.0/contact) + 2D Compatibility (PS) -26153 + (NN) -2740 + (LL) 3152 1D Compatibility (HY) -14800 + (ID) 4300 Total energy: -176107.0 ( -89.94 by residue) QMean score : 0.366

(partial model without unconserved sides chains): PDB file : Tito_2X8D.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2X8D-query.scw PDB file : Tito_Scwrl_2X8D.pdb: