Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTMKQFEIAAIPGDGVGKEVVAAAEKVLHTAAEVHGGLSFSFTAFPWSCDYYLEHGKMMPEDGIHTLTQFEAVFLGAVGNPKLVPDHISLWGLLLKIRRELELSINMRPAKQMAGITSPLLHPNDFDFVVIRENSEGEYSEVGGRIHRGDDEIAIQNAVFTRKATERVMRFAFELAKKRRSHVTSATKSNGIYHAMPFWDEVFQQTAADY--SGIETSSQHIDALAAFFVTRPETFDVIVASNLFGDILTDISSSLMGSIGIAPSANINPSGKYPSMFEPVHGSAPDIAGQGLANPIGQIWTAKLMLDHFGE-------EELGAKILDVMEQVTADG-IKTRDIGGQSTTAEVTDEICSRLRKL 4Y1P Chain:B ((3-336)) -----FVVALIQGDGIGPEVVSKSKTILARLNEKFS-LPIEYIEVEAGDTTKNKFGDALPKDSLRVIEKADMILKGPVGE--------TAADVVVKLRLMYDLYANLRPAKSLPGLENKF---GDVDILVVRENTEDLYK---GLEHVISDGVTVGIKVITRAASTRIAQVALNQALRRKKKVVCVHKSN----VMRITDGLFAESCRNVLKGKVEYSEMYVDAAAANLVRNPQAFDVIVTENTYGDILSDEAGQIAGSLGISPSANI---GDRKSLFEPVHGAAFDIAGKNIANPTAFLLSVGMMLDRMQELSGDIRYNNAAKSLRDAIYSVYSEGKYLTPDVGGSSTTDEMISAIRSKI---

General information: TITO was launched using: RESULT: Template: 4Y1P.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 1862 -162329 -87.18 -501.01 target 2D structure prediction score : 0.69 Monomeric hydrophicity matching model chain B : 0.77 3D Compatibility (PKB) : -87.18 2D Compatibility (Sec. Struct. Predict.) : 0.69 1D Compatibility (Hydrophobicity) : 0.77 QMean score : 0.507

(partial model without unconserved sides chains): PDB file : Tito_4Y1P.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4Y1P-query.scw PDB file : Tito_Scwrl_4Y1P.pdb: