Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MHCAENCIFCKIIAGDIPSAKVYEDEHVLAFLDISQVTKGHTLVIPKTHIENVYEFTDELAKQYFHAVPKIARAIRDEFEPIGLNTLNNNGEKAGQSVFHYHMHIIPRYGKGDGFGAVWKTHADDYKPEDLQNISSSIAKRLASS 3KSV Chain:A ((11-148)) ----ANCIFCKIIKGDIPCAKVAETSKALAFMDINPLSRGHMLVIPKEHASCLHELGMEDAADVGVLLAKASRAVAGPDGSMQYNVLQNNGSLAHQEVPHVHFHIIPKTDEKTGLKIGWDTV--KVASDELAEDAKRYSEAIAK-

General information: TITO was launched using: RESULT: Template: 3KSV.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 481 -73516 -152.84 -532.72 target 2D structure prediction score : 0.55 Monomeric hydrophicity matching model chain A : 0.79 3D Compatibility (PKB) : -152.84 2D Compatibility (Sec. Struct. Predict.) : 0.55 1D Compatibility (Hydrophobicity) : 0.79 QMean score : 0.432

(partial model without unconserved sides chains): PDB file : Tito_3KSV.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3KSV-query.scw PDB file : Tito_Scwrl_3KSV.pdb: