Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MAERMVGKQAPRFEMEAVLASKEFGKVSLEENMKNDKWTVLFFYPMDFTFVCPTEITAMSDRYDEFEDLDAEVIGVSTDTIHTHLAWINTDRKENGLGQLKYPLAADTNHEVSREYGVLIEEEGVALRGLFIINPEGELQYQTVFHNNIGRDVDETLRVLQALQ----TGGLCPANWKPGQKTL 3SBC Chain:G ((25-200)) -----VQKQAPTFKKTAVV-DGVFDEVSLDK--YKGKYVVLAFIPLAFTFVSPTEIIAFSEAAKKFEEQGAQVLFASTDSEYSLLAWTNIPRKEGGLGPINIPLLADTNHSLSRDYGVLIEEEGVALRGLFIIDPKGVIRHITINDLPVGRNVDEALRLVEAFQWTDKNGTVLPCNWTPGAATI

General information: TITO was launched using: RESULT: Template: 3SBC.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain G - contact count / total energy / energy per contact / energy per residue : 896 -118408 -132.15 -688.42 target 2D structure prediction score : 0.60 Monomeric hydrophicity matching model chain G : 0.83 3D Compatibility (PKB) : -132.15 2D Compatibility (Sec. Struct. Predict.) : 0.60 1D Compatibility (Hydrophobicity) : 0.83 QMean score : 0.580

(partial model without unconserved sides chains): PDB file : Tito_3SBC.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3SBC-query.scw PDB file : Tito_Scwrl_3SBC.pdb: