Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSINIKAVTDDNRAAILDLHVSQNQLSYIES----TKVCLEDA-KEC-HYYKPVGLYY--------EGDLVGFAMYGLFPEYDEDNKNGRVWLDRFFIDERYQGKGLGKKMLKALIQHLAELYKCKRIYLSIFENNIHAIRLYQRFGFQFNGELDFNGEKVMVKEL 2B5G Chain:A ((2-152)) AKFVIRPATAADCSDILRLIKELAKYE----QVILTEKDLLEDGFGEHPFYHCLVAEVPKEHWTPEGHSIVGFAMYYFTYDP---WIGKLLYLEDFFVMSDYRGFGIGSEILKNLSQVAMRC-RCSSMHFLVAEWNEPSINFYKRRGASDLSSEE-----------

General information: TITO was launched using: RESULT: Template: 2B5G.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 511 -27056 -52.95 -203.42 target 2D structure prediction score : 0.52 Monomeric hydrophicity matching model chain A : 0.66 3D Compatibility (PKB) : -52.95 2D Compatibility (Sec. Struct. Predict.) : 0.52 1D Compatibility (Hydrophobicity) : 0.66 QMean score : 0.511

(partial model without unconserved sides chains): PDB file : Tito_2B5G.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2B5G-query.scw PDB file : Tito_Scwrl_2B5G.pdb: