Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MAKLDETLTMLKDLTDAKGIPGNEREVRQVMKSYIEPFADEVTTDRLGSLIAKKTGAENGPKIMIAGHLDEVGFMVTQITDKGFIRFQTVGGWWAQVMLAQRVTIVTKKGEITGVIGSKPPHILSPEARKKSVEIKDMFIDIGASSREEALEWGVLPGDMIVPHFEFTVMNNEKFLLAKAWDNRIGCAIAIDVLRNLQN-T-DHPNIVYGVGTVQEEVGLRGAKTAAHTIQPDIAFGVDVGIAGDTPGISEKEAQSKMGKGPQIIVYDASMVSHKGLRDAVVATAEEAGIPYQFDAIAGGGTDSGA--IHLTANGVPALSITIATRYIHTHAAMLHRDDYENAVKLITEVIKKLDRKTVDEITYQ 5DS0 Chain:A ((2-359)) --NADKSMELMKTLMEAFGPSGFEREVNAICKEYMEPYADEVVVDKLGSVTFIAKGN-DRPRILMAGHTDEVGFIVSSISKEGYLTFNTLGGWWSQVLLGQRVVVRTCKGMVHGIIASKPPHILPPDERKKIVEARDMFIDIGATSEEEAEESGVKVGDPIVPWSPFSVIQNGRVAMGKAFDDRIGAFVLMEAIRRMKDQGIEHPNTVYGSATVQEEVGLRGAQTTAHVVDPDVALVLEVDIAGDVP--KPHEALTKMGKGPGLVTYDRSMIPNQPLKEFVINVAKQAQIPLQLSQMSGGGTDAGRIHMN--RAGCPSVVITIPTRHIHSHVGLLSLKDTENAIRLVIELIKRLDLETVEGFT--

General information: TITO was launched using: RESULT: Template: 5DS0.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 2138 -134214 -62.78 -381.29 target 2D structure prediction score : 0.60 Monomeric hydrophicity matching model chain A : 0.86 3D Compatibility (PKB) : -62.78 2D Compatibility (Sec. Struct. Predict.) : 0.60 1D Compatibility (Hydrophobicity) : 0.86 QMean score : 0.594

(partial model without unconserved sides chains): PDB file : Tito_5DS0.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5DS0-query.scw PDB file : Tito_Scwrl_5DS0.pdb: